An Animal Model Using Metallic Ions to Produce Autoimmune Nephritis

Figure 8

Double fluorescence assay in an animal treated with CuSO₄ showing (a) a glomerulus stained in green by FITC-annexin localized to the apoptotic membranes, (b) La ribonucleoprotein stained by Texas red, (c) glomerulus nuclei stained in blue by DAPI, (d) merging image which shows colocalization of La/annexin; the arrows are representative examples of fields analyzed for colocalization of annexin (green) and La ribonucleoprotein (red) onto apoptotic membranes. The squares correspond to magnification areas pointed by arrows.

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