Mesenchymal Stem Cells from Patients with Rheumatoid Arthritis Display Impaired Function in Inhibiting Th17 Cells
Protein array analysis of cytokine profiles in RA MSCs. After culture for 24 hours, MSC supernatant was collected and analyzed using a cytokine protein array. (a) The images of spot signal on the membrane due to each cytokine are shown. Detailed information of the cytokine protein array used in this study was shown in the supplementary material (Figure S3). (b) Histogram of several cytokines measured by the protein array. For data set of each cytokine, left column represents control MSCs and right column represents RA MSCs. (c–e) Levels of IL-6, CCL2, and RANTES assayed by ELISA. n.s., no significant difference. IGFBP, insulin-like growth factor binding protein; SCF, stem cell factor; SDF-1, stromal cell-derived factor-1; RANTES, the regulated upon activation normal T cell expressed and secreted, also called CCL5.