Secretion of S100A8, S100A9, and S100A12 by Neutrophils Involves Reactive Oxygen Species and Potassium Efflux

Figure 4

Phagocytic particles induce the secretion of S100A8/A9 and S100A12. Neutrophils were stimulated with 1.5 mg/mL MSU crystals or fullerenes for increasing periods of time. (a) S100A8/A9 and (b) S100A12 in the supernatants were then quantified by ELISA. (c and d) Neutrophils were stimulated with 1.5 mg/mL MSU crystals, 1.5 mg/mL TiO₂, 3 mg/mL silica, 1.5 mg/mL carbon nanotubes, or 1.5 mg/mL fullerenes for 60 minutes. (c) S100A8/A9 and (d) S100A12 in the supernatants were then quantified by ELISA. Results represent the means ± SEM of 5 donors. ; .

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