Secretion of S100A8, S100A9, and S100A12 by Neutrophils Involves Reactive Oxygen Species and Potassium Efflux

Figure 6

Inhibition of calgranulin secretion by glibenclamide. Neutrophils were preincubated with the ATP-sensitive K⁺ channel inhibitor glibenclamide (50 μM) and then stimulated with 1.5 mg/mL MSU crystals or 10 nM PMA for 60 minutes. (a) S100A8/A9 and (b) S100A12 in the supernatants were then quantified by ELISA. Results represent the means ± SEM of 5 donors. ; .

(a)

(b)