Secretion of S100A8, S100A9, and S100A12 by Neutrophils Involves Reactive Oxygen Species and Potassium Efflux

Table 1

Proteomic analysis of proteins secreted by neutrophils.

Accession number	Name	Number of peptides	MSU/fMLP	value	Fullerenes/fMLP	value
NETosis-associated proteins
P04040	Catalase	26	2.46		1,123677
P16401	Histone H1.5	3	1,020058	0,973432	0,745488	0,050647
Q99880	Histone H2B type 1-L	3	0,51384	0,677915	0,386307	0,237565
Granule’s antimicrobial proteins and enzymes
P14780	Metalloproteinase-9	27	1,632532	0,285746	0,757033	0,475546
P05164-3	Myeloperoxidase	46	4,544349		0,934431	0,071378
P08246	Neutrophil elastase	8	4,532902	0,001009	1,151382	0,1147
P02788	Lactoferroxin-C	70	5,713208	0,014768	1,489547	0,117136
O75594	Peptidoglycan recognition protein 1	6	3,00764	0,00035	0,892349	0,189002
Cytoplasmic/intracellular proteins
P07195	Lactate dehydrogenase B chain	3	1,563257	0,274256	1,324116	0,095393
P04083	Annexin A1	6	1,199672	0,307777	0,695102	0,001041
P12429	Annexin A3	3	1,917971	0,038612	0,974026	0,701223
P26447	S100A4	3	1,885171	0,033789	1,114386	0,027142
P31949	S100A11	2	1,926023	0,159743	1,063338	0,363006
P35579	Myosin-9	45	1,276348	0,097059	1,114768
Q53GK6	Beta actin variant	12	1,649348	0,235332	1,09936	0,612781
Calgranulins
P05109	S100A8	15	5,706729		2,008216	0,001211
P06702	S100A9	17	5,775399		1,982834	0,000234
P80511	S100A12	7	2,426018	0,042156	1,293581	0,001423

Number of peptides identified by isobaric tags for relative and absolute quantitation.
Ratio of the different proteins in supernatants from neutrophils stimulated with MSU crystals over neutrophils stimulated with fMLP (which does not induce secretion of calgranulins).
value for the identification of the peptides.
Ratio of the different proteins in supernatants from neutrophils stimulated with fullerenes over neutrophils stimulated with fMLP (which does not induce secretion of calgranulins).