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Journal of Immunology Research
Volume 2015, Article ID 316351, 8 pages
http://dx.doi.org/10.1155/2015/316351
Research Article

Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors

1Department of Hematology, The People’s Hospital of Bozhou, Bozhou 236800, China
2Department of Hematology, First Hospital of Quanzhou Affiliated to Fujian Medical University, Quanzhou 362000, China

Received 14 April 2015; Revised 12 June 2015; Accepted 14 July 2015

Academic Editor: Menaka C. Thounaojam

Copyright © 2015 Mei Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mesenchymal stem cells are immunoregulation cells. IL-22 plays an important role in the pathogenesis of immune thrombocytopenia. However, the effects of mesenchymal stem cells on IL-22 production in patients with immune thrombocytopenia remain unclear. Flow cytometry analyzed immunophenotypes of mesenchymal stem cells; differentiation of mesenchymal stem cells was observed by oil red O and Alizarin red S staining; cell proliferation suppression was measured with MTS; IL-22 levels of cell-free supernatants were determined by ELISA. Mesenchymal stem cells inhibited the proliferation of activated CD4+T cells; moreover, mesenchymal stem cells immunosuppressed IL-22 by soluble cellular factors but not PGE2. These results suggest that mesenchymal stem cells may be a therapeutic strategy for patients with immune thrombocytopenia.