Development of Nonaggregating Poly-A Tailed Immunostimulatory A/D Type CpG Oligodeoxynucleotides Applicable for Clinical Use

Figure 3

Physical properties of polynucleotide tailed A/D type ODNs in PBS solution. DLS analysis of the indicated ODN solutions (each 1.0 mg/mL in PBS) by % intensity plot (a) or % mass plot (b). See Table 2 for each measurement. (c) Turbidity status of the indicated ODN solution. The indicated ODNs were initially dissolved in distilled water at a concentration of 10 mg/mL (all ODNs were completely solubilized with water and the solutions were clear) and further diluted with PBS at a final concentration of 1.0 mg/mL. Solutions were stored at 4°C for at least 18 hours and then images were captured. D35 developed visible white turbidity during this incubation. In contrast, other ODNs were clear. (d) TEM image of aggregated D35 in PBS similarly prepared as in (c).

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