Figure 3: Enhancement of ASC formation by non-CpG ODN. Cells were incubated with the different combinations of ODNs and OVA as indicated; then after 4 days the cultures were transferred onto nitrocellulose-covered slides coated with light chain capture antibodies. After 10 hours of incubation, slides were washed and IgM (a) and IgG (b) spots produced by ASCs were detected using labeled antibodies and a fluorescent scanner. A representative fluorescent spot experiment is shown in (c). Statistical significance was calculated using two-tailed permutation test. Treatment pairs with significant differences () are indicated by connecting brackets; thin lines for within OVA treatment group comparisons and thick lines for OVA treatment effects. ASC: antibody secreting cell.