Figure 4: No synergism between OVA and ODN treatments when cognate T cells are not present. Wild type (wt) or TCR transgenic (tg) splenic cells were incubated with different combinations of ODN and OVA for 1 day ((a), (b)) or 4 days (c). After 1 day CD69 expression was measured on B and T cell populations by flow cytometry ((a), (b)). Cells from the 4-day cultures were washed and then transferred onto nitrocellulose-covered slides that were previously coated with light chain capture antibodies. After 10 h of incubation, slides were washed and spots indicating IgG producing ASCs were detected using fluorescently labeled antibodies and a fluorescent scanner (c). Results represent a single experiment.