Effective Binding of a Phosphatidylserine-Targeting Antibody to Ebola Virus Infected Cells and Purified Virions

Figure 1

Flow cytometry staining of EBOV-infected cells. (a) Single color staining of cells with anti-EBOV, Erbitux, and PGN401 antibodies. Histograms show frequency of cells versus level of fluorescence intensity. The marker regions quantify the percentage of cells stained above background levels. (b) Dual color labeling with anti-EBOV and PGN401 antibodies. The -axis relates to the detection of the FL1 channel that detects Alexa-Fluor 488 staining (anti-mouse detector for EBOV antibody) and the -axis, the FL4 channel that detects Alexa Fluor 647 staining (anti-human detector for PGN401 and Erbitux). Quadplots identify the percentage of cells within each region.

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