Differential Immune Response against Recombinant Leishmania donovani Peroxidoxin 1 and Peroxidoxin 2 Proteins in BALB/c Mice
Cytokine responses in spleen cells of rLdPxn1 and rLdPxn2 immunized mice. Mice were immunized s.c. three times at three-week intervals with rLdPxn1 or rLdPxn2 with or without CpG ODN or GLA-SE. Four weeks after the last immunization, spleen cells were prepared and in vitro stimulated with the respective antigen (2 and 10 g/mL) or ConA (5 g/mL). The release of IFN- (ng/mL), IL-10 (ng/mL), and IL-4 (pg/mL) in immunized mice was measured in supernatants after 72 hr of in vitro stimulation at 37°C. Results are presented as the amount of IFN- (ng/mL) and IL-10 (ng/mL) for rLdPxn1 (a) and rLdPxn2 (b) or the amount of IL-4 (pg/mL) of rLdPxn1 and rLdPxn2 (c).