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Journal of Immunology Research
Volume 2015, Article ID 352934, 13 pages
Research Article

Age-Related Differences in Percentages of Regulatory and Effector T Lymphocytes and Their Subsets in Healthy Individuals and Characteristic STAT1/STAT5 Signalling Response in Helper T Lymphocytes

1Department of Allergology, Rheumatology and Clinical Immunology, University Children’s Hospital, University Medical Centre Ljubljana, Bohoričeva 20, SI-1525 Ljubljana, Slovenia
2Department of Laboratory Diagnostics, University Medical Centre Maribor, Ljubljanska Ulica 5, SI-2000 Maribor, Slovenia
3Faculty of Medicine, University of Maribor, Taborska Ulica 8, SI-2000 Maribor, Slovenia
4Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, SI-1000 Ljubljana, Slovenia
5Department of Pediatrics, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, SI-1000 Ljubljana, Slovenia

Received 29 March 2015; Revised 6 August 2015; Accepted 27 August 2015

Academic Editor: Aurelia Rughetti

Copyright © 2015 Marija Holcar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Table S1. Concentrations of remaining studied cell subsets in all three age groups of healthy subjects analyzed by flow cytometry (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). Calculated concentrations of the T lymphocyte subsets complement information showed by the percentages. There is similar significant increase in the concentrations of all different Teff subsets whereas the concentrations of the rTreg subset which is group of cells that is the most supressive in vivo significantly drops with age.Together this data demonstrate a significant shift towards an effector T cell phenotype as children become adults.

Additional file 2: Figure S1. pSTAT5 after IL-2 stimulation in Th lymphocytes depends on the levels of IL-2Ralpha. Whole blood samples were stimulated with IL-2, stained with anti-CD25 antibodies and prepared for analysis of Th cell STAT5 signalling as described in materials and methods. Representative plots of gated lymphocytes (A) and CD4+ Th cells (B) from healthy donors are shown. The small boxes represent individual bins within the distribution of CD25 (IL-2Ralpha) and CD4. Within each bin, the geometric mean fluorescence intensity of the response channel (pSTAT5) was calculated for display using ScatterSlice software: pSTAT5 for varying levels of IL-2Ralpha and CD4 as defined by colour code shown on the right.

  1. Supplementary Material