Journal of Immunology Research / 2015 / Article / Tab 1

Research Article

Osteoprotegerin Polymorphisms in a Mexican Population with Rheumatoid Arthritis and Generalized Osteoporosis: A Preliminary Report

Table 1

Genotyping strategies for TNFRSF11B polymorphism variants detection.

SNPPrimersBand size (bp)Restriction enzymeRecognized sequenceBand size after digestion (bp)

C950T
rs2073617
5′-TGCGTCCGGATCTTGGCTGGATCGG-3′548 Hinc II GTY RACC 248 and 287
T 548
5′-ACTTACCACGAGCGCGCAGCACAGCAA-3′

C209T
rs3134070
5′-CGAACCCTAGAGCAAAGTGC-3′271 Taq I T CGAC 212, 31 and 28
T 212 and 59
5′-TGTCTGATTGGCCCTAAAGC-3′

T245G
rs3134069
5′-CGAACCCTAGAGCAAAGTGC-3′271 Hinf I G ANTCA 195, and 176
C 127, 76 and 69
5′-TGTCTGATTGGCCCTAAAGC-3′

SNP: single nucleotide polymorphism and bp: base pair.

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