Enhanced Cysteinyl-Leukotriene Type 1 Receptor Expression in T Cells from House Dust Mite-Allergic Individuals following Stimulation with Der p

Figure 4

Functional analysis of increased CysLT₁ expression on T cells. The biological significance of the enhanced CysLT₁ receptor expression on T cells was assessed by measuring intracellular calcium flux in response to LTD₄. PBMC from HDM-allergic (HDMA) individuals were exposed for 72 h to Der p in the presence or absence of neutralizing Abs to IL-4 or IL-10 or of the CysLT₁ receptor antagonist MK571. Cells were then labeled with anti-CD3 and anti-CD4 Ab and loaded with Fluo4-AM for the calcium mobilization assay on a FACSCalibur flow cytometer. (a) Raw data representative of 4 separate experiments. (b) Compiled data of peak calcium responses. ; values indicated above histograms, with respect to the vehicle.

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(b)