Vaccination with an Attenuated Ferritin Mutant Protects Mice against Virulent Mycobacterium tuberculosis

Figure 1

Experimental design, lung bacillary burden, and gross pathology of vaccinated and uninfected or Mtb-infected mice. (a) Schema of the mice vaccination schedule and Mtb infection experiment. (b) Lung bacillary load in mice infected with virulent Mtb H37Rv after vaccination with PBS (sham) or BCG or bfrB for 4 () or 8 weeks (). Gross lung pathology of vaccinated and uninfected or Mtb-infected-mice. (c) H&E stained lung section of BCG vaccinated (for 8 weeks) and uninfected mice. (d) H&E stained lung section of bfrB vaccinated (for 8 weeks) and uninfected mice. (e) H&E stained lung section of BCG vaccinated (for 8 weeks) and Mtb-infected (for 4 weeks) mice. The arrows in (e) show a multifocal, coalescent granuloma. (f) H&E stained lung section of bfrB vaccinated (for 8 weeks) and Mtb-infected (for 4 weeks) mice. The arrows in (f) show multiple, small granulomas.

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