Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiri

Figure 7

Fyn detection in stimulated human PBMC. (a) PBMC were treated with PHA-P and hTip-CSKH during 2 h, lysed, and the protein extract resolved by SDS-PAGE. Western blot was performed with an anti-Fyn specific antibody. PHA-P was used as positive control, which induces the appearance of a second band of 56 kDa. (b) Quantification of the Fyn p59 and p56 bands. The intensity of each Fyn band was normalized to the loading control β-actin.

(a)

(b)