Evidence for Contribution of CD4+CD25+ Regulatory T Cells in Maintaining Immune Tolerance to Human Factor IX following Perinatal Adenovirus Vector Delivery

Figure 2

Adoptive transfer of regulatory cell populations, tissue distribution of vector, and transgene expression. (a) Anti-hFIX antibody concentrations in serum diluted 1/100 following adoptive transfer of lymphocytes from hFIX-sensitised mice (black bar), cotransplantation of sensitised cells plus CD4+CD25+ cells from neonatally tolerized mice (blue bar) or plus CD4+CD25− cells from neonatally tolerized mice (red bar), or adoptive transfer of lymphocytes from naive donors (grey bars). Recipients underwent sublethal irradiation, adoptive transfer, and hFIX-immunisation 24 hours later and blood collection on days 7, 17, and 24. (b) Quantitative PCR analysis of vector genome distribution and (c) luciferase expression six weeks after neonatal injection of AdLuc.

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