RNA 1 and RNA 2 Genomic Segments of Chronic Bee Paralysis Virus Are Infectious and Induce Chronic Bee Paralysis Disease

Table 2

Sequences of the primers and probes used for the CBPV RNA 2 RT-qPCR. Oligonucleotide sequences are located on RNA 2 complete genome of the A-79P isolate (NC_010712.1).

Equimolar mixture	Sequences	Position on CBPV-RNA 2; (based on A79-P isolate)
Forward primer mix	AGGCGCCGTAGCTGTTTCT	590–608 nt^1
	GCGCCGTGGCTGTTTCT	592–608 nt
Reverse primer mix	CCCCGATCATATAAGCAAACTTCTC	637–663 nt
	CCCGATCATATAGGCAAACTTCTC	639–662 nt
	CCCCGATCATATATGCAAACTTCTC	639–663 nt
Probe mix	CTGCGGTACTCAGCTCAGCTCGACG	610–634 nt
	TGCGGTACTCAGCTCAGCTCGGC	611–631 nt

¹nt: nucleotide.