Activation-Inactivation Cycling of Rab35 and ARF6 Is Required for Phagocytosis of Zymosan in RAW264 Macrophages

Figure 3

Rab35 regulates phagosome formation. (a) Effect of active and inactive Rab35 mutant expression on zymosan phagocytosis. RAW264 cells expressing each construct indicated were incubated with zymosan and then fixed. The efficiency of phagocytosis was calculated based on 50 transfected and 50 untransfected cells. The results were expressed as a percentage of control (untransfected) cells. The data are expressed as the means ± SEM of four independent experiments. and compared to GFP transfected cells (Student’s -test). (b) Effect of Rab35 knockdown on phagocytosis of zymosan. RAW264 cells stably expressing RAB35 shRNA or GFP shRNA were incubated with zymosan and were fixed. The efficiency of phagocytosis was calculated based on 50 cells expressing each Rab35 shRNA and 50 control cells stably transfected with GFP shRNA or empty shRNA vector. The results are expressed as a percentage of control cells stably transfected with empty shRNA plasmid. The data are expressed as the means ± SEM of four independent experiments. compared to GFP transfected cells (Student’s -test).

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