NKG2D Signaling Leads to NK Cell Mediated Lysis of Childhood AML

Figure 3

(a) Cytolysis of isolated NK cells comparing donor A (B content score 0) versus donor B (B content score 3) towards 14 primary childhood AML blasts at E : T ratio 10 : 1. PBMCs were isolated from peripheral whole blood from two healthy volunteer donors A and B by density gradient centrifugation using Biocoll separating solution (Biochrom) followed by a two-step immunomagnetic CD56⁺ enrichment and subsequent CD3⁺ depletion. Donor B showed significantly higher lysis than donor A (, ). (b) The analysis of blast lysis of blasts homozygous for C2 showed by trend a higher lysis of donor B (, ) applying the E : T ratio 10 : 1. Donor A was KIR2DL1 positive and donor B was KIR2DL1 negative. KIR2DL1 is the corresponding KIR receptor for the C2 HLA group.

(a)

(b)