In Vitro and In Vivo Comparison of Lymphocytes Transduced with a Human CD16 or with a Chimeric Antigen Receptor Reveals Potential Off-Target Interactions due to the IgG2 CH2-CH3 CAR-Spacer

Figure 2

Cytotoxic activity of NK- and NK-. (a) Spontaneous (NK) activity of NK-92 before and after transduction (E/T ratio: 30/1). (b) The effector cells NK- and NK- were tested against the HER2 positive BT474 cell line preincubated in the presence of increasing concentration of trastuzumab (mean of two experiments). (c) Cytotoxic activity of NK-, NK-, and NK- and against HER2 negative (MBA-MB-468) or HER2 positive (BT474) cell lines (trastuzumab, 10 mg/mL, E/T ratio: 30/1). Cytotoxicity was evaluated from release after 4 hours of incubation. The data represent the means from three independent experiments.

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