In Vitro and In Vivo Comparison of Lymphocytes Transduced with a Human CD16 or with a Chimeric Antigen Receptor Reveals Potential Off-Target Interactions due to the IgG2 CH2-CH3 CAR-Spacer

Figure 5

Tissue distribution of NK-, NK-, and NK- in NSG mice bearing an established BT474 tumor. NK-, NK-, or NK- CSFE labelled cells () were injected into NSG mice bearing BT474 tumors (tumor size range of 50 to 100 mm³). Twenty-four hours later, mouse blood, liver, tumor, lung, and spleen were collected, and the percentages of CD45 positive cells were analyzed by FACS analysis (percentage of CD45 was calculated from 50,000 events, and the means of two independent experiments are presented).

(a) Tumor

(b) Blood

(c) Spleen

(d) Liver