The Ability of Secretory Leukocyte Protease Inhibitor to Inhibit Apoptosis in Monocytes Is Independent of Its Antiprotease Activity

Figure 2

Effect of SLPI on TNF-α-induced caspase-3 and caspase-7 activity in peripheral blood monocytes. Peripheral blood monocytes were isolated and pretreated for 1 hr with SLPI (10 μg/mL) and then incubated with TNF-α (10 ng/mL) for 4 hr. Activity of the effector caspases, caspase-3 and caspase-7, was measured using the fluorogenic substrate DEVD-AMC. Results are representative of . indicates .