The Ability of Secretory Leukocyte Protease Inhibitor to Inhibit Apoptosis in Monocytes Is Independent of Its Antiprotease Activity

Figure 3

Effect of SLPI and oxidised SLPI on apoptosis in monocytic cells. U937 cells were pretreated with SLPI (10 μg/mL) or oxidised SLPI (10 μg/mL) and stimulated with TNF-α (10 ng/mL) for 4 hr. (a) Activity of the effector caspases, caspase-3 and caspase-7, was measured using the fluorogenic substrate DEVD-AMC. (b) The amount of apoptosis occurring in cells was assessed by ELISA (Roche). Results are representative of . indicates .

(a)

(b)