Journal of Immunology Research / 2015 / Article / Fig 3

Research Article

Carbohydrate Microarrays Identify Blood Group Precursor Cryptic Epitopes as Potential Immunological Targets of Breast Cancer

Figure 3

HAE3 cell surface staining detected selective expression of the HAE3-cryptic glycan markers in human cancer cell lines. (a) Four tumor cell lines, T-47D, A549, PC3, and SKMEL-28, were stained with the C1 preparation of HAE3 (IgM) at 1 : 6 dilution or with an isotype control IgM, 9.14.7 (5.0 μg/mL). (b) Seven breast cancer cell lines were stained with purified mAb HAE3 (5.0 μg/mL) or 9.14.7 (5.0 μg/mL). These cell lines are T-47D, MCF-7, SK-BR-3, BT-549, Hs578T, MDA-MB-231, and MDA-MB-468. An R-PE-conjugated goat anti-mouse IgM antibody was applied to quantify the cell surface-captured IgM antibodies. Blue line: HAE3 stain; Red line: 9.14.7 IgM isotype control.

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