Carbohydrate Microarrays Identify Blood Group Precursor Cryptic Epitopes as Potential Immunological Targets of Breast Cancer

Figure 4

A Bio-Gel P-10 plot of Tij II antigen. Tij II (20% from 2nd 10%) substance was fractioned in a precalibrated Bio-Gel P-10 column at 0.5 mL per fraction. The neutral sugar content in each fraction was determined by phenol-sulfuric acid color reaction and quantitatively measured at OD490 nm. The sizes of the Tij II substance were measured based on the neutral sugar elution profile with reference to the calibrated saccharide molecular weight standards as indicated in the plot. IM₅ stands for isomaltopentaose.