SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis

Figure 8

Luciferase reporter gene activation assays with ectopically expressed SP140L, SP140, and SP110 in HEK293 and COS-1. (a) SP140L, SP140, and SP110 fused to GAL4 DNA-binding domain were cotransfected with the luciferase reporter plasmid containing three GAL4 response elements in the promoter. The results represent the mean ± SEM of 3 independent experiments. (b) SP140L, SP140, and SP110 expression plasmids were cotransfected with glucocorticoid receptor (GR) and MMTV-Luc reporter plasmid, and the cells were treated with dexamethasone (Dex). The results represent the mean ± SEM of 3 independent experiments. NC, negative control.

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