SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis

Table 2

Primers used in the study.

Name	Application	5′ → 3′
SP140L_cDNA F1	cDNA amplification	CAAAGCCGATGGCCGGTGGGGGCA
SP140L_cDNA R1		CAGTAGCATATTTCCTGAAGGCC
SP140L_RACE R1	5′ RACE	CAGCTGGCCATCGGCTTTGC
SP140L_RACE R2		TGGTTGTTTTTCTGCGTTCTCG
SP140L F1	Cloning	TTTGAATTCATGGCAGGTGGGGGCAG
SP140L R1		TTTGTCGACTCAACTGTTCCCATTTGTTTC
SP140L R2		TTTGCGGCCGCACTGTTCCCATTTGTTTC
SP140L R3		TTTGGTACCACTGTTCCCATTTG
SP140 F1	Cloning	TTTGAATTCATGGCCCAGCAGGGCCA
SP140 R1		AAAGTCGACTCAATTGTTCCCATTTGTTTCC
SP140 R2		TTTGTCGACATTGTTCCCATTTGTTTCCTG
SP140 R3		TTTGGTACCATTGTTCCCATTTGTTTC
SP110 F1	Cloning	TTTGAATTCATGTTCACCATGACAAGAGC
SP110 R1		AAAGTCGACTCAAGGAAGAGTCCAGAA
SP110 R2		TTTGTCGACAGGAAGAGTCCAGAAACC
SP100A F	Cloning	TTTGAATTCATGGCAGGTGGGGGCGGCGA
SP100A R		TTTGGTACCATCTTCTTTACCTGACCCTCTTCTTAGG
SP140L_QPCR F1	Expression analysis	TGGAAGCACTGTTCAGCGAGGT
SP140L_QPCR R1		TGATGTCAGGCCTCTCTTCCCT
SP140_QPCR F1	Expression analysis	CCAGGTGGGGGAGTGTCCTGT
SP140_QPCR R1		TCTCCCCTGGTGCTGTGCTGT
SP110_QPCR F1	Expression analysis	CCATACCCCACTGGCGCTGC
SP110_QPCR R1		GTCAGATGGGCTGGGCGAC
SP100_QPCR F1	Expression analysis	GGCTGAGCCAACAGAGTCCTGCG
SP100_QPCR R1		TCCACCAGTCGCACAGAACAGGA
B2M_QPCR F1	Expression analysis	TGCTCGCGCTACTCTCTCT
B2M_QPCR R1		TCCATTCTCTGCTGGATGAC
HPRT_QPCR F1	Expression analysis	GACTTTGCTTTCCTTGGTCAGG
HPRT_QPCR R1		AGTCTGGCTTATATCCAACA CTTCG