Journal of Immunology Research / 2015 / Article / Fig 3

Research Article

Vitamin D Deficiency Contributes to the Reduction and Impaired Function of Naïve Regulatory T Cell in Chronic Heart Failure

Figure 3

Variations of Foxp3+ cell subpopulations under physiological and CHF conditions. (a) Flow cytometry of PBL gated on CD4+T cells isolated from PBL. A schematic of T cell subpopulations separated based on Foxp3 and CD45RA. (b) Flow cytometry of PBL gated on CD4+T cells isolated from a healthy adult, an old donor, and three patients from each clinical stage. Percentage of each quadrant in each staining combination is shown. (c) Percentages of each Foxp3+ subset among CD4+T cells in indicated numbers of patients with CHF NYHA II/III/IV, AMI, aged donors (between 79 and 90 years old), and healthy donors (between 18 and 60 years old). Black horizontal bars represent mean percentage. Data represent the mean ± SEM; *, **, and ***.
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