Vitamin D Deficiency Contributes to the Reduction and Impaired Function of Naïve
Regulatory T Cell in Chronic Heart Failure
The impact of 1,25(OH)2D on T cell. (a) PBMCs purified from HD were activated by CD3/CD28 with 20 nM 1,25(OH)2D or DMSO. Cells were collected on days 1, 3, and 5 and analyzed apoptosis in CD4+T cell by FACS. (b) PBMCs were cultured as described with a series of concentrations of 1,25(OH)2D from 10 nM to 100 nM and observed apoptosis in CD4+T cell on day 3. (c) CD4+T cell purified from HD were activated by αCD3/CD28 monoclonal antibodies with 20 nM 1,25(OH)2D or DMSO. Cells were collected on days 1, 3, and 5 and analyzed CD45RA expression by FACS. (d) CD4+T cell from CHF was cultured as described with a series of concentrations of 1,25(OH)2D from 10 nM to 100 nM and observed CD45RA expression. (e) CD45RA and Foxp3 expressions in CD4+T cell with or without vitamin d supplement on day 3. Data are representative of three independent experiments.
We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.