Journal of Immunology Research / 2015 / Article / Fig 4

Research Article

Vitamin D Deficiency Contributes to the Reduction and Impaired Function of Naïve Regulatory T Cell in Chronic Heart Failure

Figure 4

The impact of 1,25(OH)2D on T cell. (a) PBMCs purified from HD were activated by CD3/CD28 with 20 nM 1,25(OH)2D or DMSO. Cells were collected on days 1, 3, and 5 and analyzed apoptosis in CD4+T cell by FACS. (b) PBMCs were cultured as described with a series of concentrations of 1,25(OH)2D from 10 nM to 100 nM and observed apoptosis in CD4+T cell on day 3. (c) CD4+T cell purified from HD were activated by αCD3/CD28 monoclonal antibodies with 20 nM 1,25(OH)2D or DMSO. Cells were collected on days 1, 3, and 5 and analyzed CD45RA expression by FACS. (d) CD4+T cell from CHF was cultured as described with a series of concentrations of 1,25(OH)2D from 10 nM to 100 nM and observed CD45RA expression. (e) CD45RA and Foxp3 expressions in CD4+T cell with or without vitamin d supplement on day 3. Data are representative of three independent experiments.
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