Vitamin D Deficiency Contributes to the Reduction and Impaired Function of Naïve Regulatory T Cell in Chronic Heart Failure

Figure 7

1,25(OH)2D ameliorates the impaired immunomodulation of CD4⁺CD45RA⁺T cell. (a) CD4⁺CD45RA⁺T cells were purified from HD and CHF, labeled with CFSE subsequently, and incubated with 1,25(OH)2D or DMSO for 5 days. Representative flow cytometric dot plots and histograms of CFSE and IL-17A double staining. (b) Suppression of responder T cell proliferation was preceded using transwells. 1,25(OH)2D induced CD4⁺CD45RA⁺T or CD4⁺CD45RA⁻T cells were placed into top chamber while  CD4⁺CD25⁻T cells were added into bottom chamber. After 5 days, cells were stained for CD4 and proliferation of CFSE-labeled responder T cells was analyzed on a flow cytometer. (c) The level of IL-10 and IL-17 in culture supernatant on day 5 was detected by ELISA. Data are representative of five independent experiments. Results shown are the mean ± SEM; ^*.

(a)

(b)

(c)