Research Article

A Humanized Anti-CD22-Onconase Antibody-Drug Conjugate Mediates Highly Potent Destruction of Targeted Tumor Cells

Figure 4

Purification of SPDP-based ADCs with distinct molar DAR. Ion exchange chromatography yielded immuno-RNases with distinct OARs as confirmed by SDS-PAGE under nonreducing (a) and reducing (c) conditions. Lane 1: molecular weight marker; lanes 2–4: ADCs with OARs of 1 : 1, 2 : 1, and 3 : 1 (1 μg each); lane 5: huRFB4 IgG (1 μg); lane 6: ONC (1 μg). (b) Size exclusion chromatography of IEX-purified immuno-RNases was performed on a Superdex 200 10/300 GL column.