Acceleration of Wound Healing by -gal Nanoparticles Interacting with the Natural Anti-Gal Antibody

Figure 2

Recruitment of macrophages by 10 mg α-gal nanoparticles injected intradermal in anti-Gal producing GT-KO mice. (a) Macrophages migrating toward the injection site, 24 h after injection. The injection site is indicated as the empty area since the α-gal nanoparticles are dissolved by the alcohol fixative (H&E ×100). (b) The injection site after 4 days immunostained with macrophage specific HRP-anti-F4/80 Ab. The recruited cells are stained by this Ab (×200). (c) The injection site after 7 days demonstrating many large macrophages with ample cytoplasm (H&E ×400). (d) Individual macrophages isolated on day 7 displaying large size and multiple cytoplasmic vacuoles, probably the result of activation and uptake of the anti-Gal coated α-gal nanoparticles (Wright staining ×1000). Modified from [19].

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