Gingiva Equivalents Secrete Negligible Amounts of Key Chemokines Involved in Langerhans Cell Migration Compared to Skin Equivalents

Table 1

Summary of results obtained after exposing skin and gingiva tissue equivalents to TNF, an allergen (CA), or an irritant (SDS).

Experimental condition	Dose response within tissue^a		Dose response between tissues SE > GE^b	2 comparisons between tissues^c
	Skin	Gingiva
Proinflammatory cytokines
IL-18
TNF	ns	ns	ns	ns
SDS	ns	ns	ns	ns
CA		ns		SE > GE
CCL27
TNF		ns	ns	ns
SDS	ns	ns		SE > GE
CA	ns^d	ns		SE > GE
LC migration chemokines
CCL2
TNF			ns	SE > GE
SDS	ns	ns	ns	ns
CA	ns	ns	ns	ns
CCL5
TNF	ns	ns	ns	ns
SDS	ns	ns	ns	ns
CA	ns	ns	ns	ns
CCL20
TNF		ns	ns	SE > GE
SDS		ns		SE > GE
CA	ns	ns	ns	ns
CXCL12
TNF		ns		SE > GE
SDS	ns	ns		SE > GE
CA	ns	ns		SE > GE
General inflammatory mediators
CXCL8
TNF			ns	ns
SDS	ns		ns	ns
CA	ns	ns	ns	ns
IL-6
TNF			ns	ns
SDS	ns		ns	ns
CA	ns		ns	ns (0.06 GE > SE)

Dose response within tissue: statistical significance of differences between the unexposed and exposed equivalents, KC, or fibroblasts was calculated using a paired -test.
^bDose response between tissues: SE > GE. For comparisons between dose responses of skin and gingiva, an unpaired -test was used.
^cTwo single comparisons between tissues (unpaired -test).
^dSkin/CCL27: significant difference between 0 and 20 mM CA (; paired -test) but not in entire dose response.
Tests used 2-way ANOVA followed by Dunnett’s multiple comparison using GraphPad version 6.0, Data represent the average of at least 3 individual experiments ± SEM ; ; .