Experimental Autoimmune Encephalomyelitis Development Is Aggravated by Candida albicans Infection

Figure 4

Modulation of MOG-induced cytokine production by previous infection with C. albicans. C57BL/6 mice were infected with C. albicans and 3 days later they were submitted to EAE induction. Fourteen days after EAE induction, some immunological parameters were evaluated in the spleen. The percentage of CD3⁺CD4⁺ (a) and CD3⁺CD4⁺CD25⁺FoxP3⁺ (b) was performed by cytometric analysis in 100.000 acquired events. IL-10 (c), TNF-α (d), IL-6 (e), IL-17 (f), IFN-γ (g), IL-2 (h), and IL-4 (i) levels were measured in spleen cell cultures stimulated with MOG or heat-killed C. albicans. The results are expressed as mean ± SD ( mice/group). ANOVA, Tukey’s test, and . Different letters indicate statistical difference among the groups (a and b) or among the groups under the same in vitro stimulation (c, d, e, f, g, h, and i).

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