The Pathogenicity of Anti-β2GP1-IgG Autoantibodies Depends on Fc Glycosylation

Figure 2

mOD values of anti-β2GP1-IgG1 (a), anti-β2GP1-IgG2 (b), anti-β2GP1-IgG3 (c), and anti-β2GP1-IgG4 (d) in NHD, PAPS, SLE/APS, and children. The horizontal lines represent the cut-off for seropositivity of the corresponding anti-β2GP IgG-subclass (>mean + 2 ×  ); NHD = normal healthy donor; PAPS = primary antiphospholipid syndrome; SLE/APS = antiphospholipid syndrome with systemic lupus erythematosus; OD = optical density. Median OD values of the anti-β2GP1-IgG subclasses in the study cohorts (e). Regarding the anti-β2GP1-IgG subclass-distribution within the cohorts, children showed a significantly higher contribution of IgG3 than IgG1 and IgG2. Patients with antiphospholipid syndrome (APS) showed a higher content of anti-β2GP1-IgG2 than -IgG1 and -IgG3. NHD showed a higher contribution of anti-β2GP1-IgG2 than -IgG1, -IgG3, and -IgG4. The columns represent the median OD values of IgG1, IgG2, IgG3, and IgG4 in the cohorts. OD = optical density; NHD = normal healthy donor. Percentage distribution of anti-β2GP1-IgG subclasses in children and patients with APS (f). Children harbour significantly less anti-β2GP1-IgG2 than patients with APS but more anti-β2GP1-IgG3. APS = antiphospholipid syndrome.

(a)

(b)

(c)

(d)

(e)

(f)