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Journal of Immunology Research
Volume 2015, Article ID 718975, 9 pages
Research Article

IL-10 and ARG-1 Concentrations in Bone Marrow and Peripheral Blood of Metastatic Neuroblastoma Patients Do Not Associate with Clinical Outcome

1Laboratorio di Oncologia IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy
2Laboratorio di Bioterapia, IRCCS AOU San Martino-Istituto Nazionale per la Ricerca sul Cancro, 16132 Genova, Italy
3Laboratorio di Patologica Clinica IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy
4U.O. Oncologia IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy

Received 1 August 2014; Accepted 25 September 2014

Academic Editor: Marco Antonio Velasco-Velázquez

Copyright © 2015 Fabio Morandi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The expression of the immunosuppressive molecules IL-10 and arginase 1 (ARG-1), and of FOXP3 and CD163, as markers of regulatory T cells (Treg) and macrophages, respectively, was evaluated in bone marrow (BM) and peripheral blood (PB) samples collected at diagnosis from patients with metastatic neuroblastoma (NB). IL-10 and ARG-1 plasma concentrations were measured and the association of each parameter with patients’ outcome was tested. The percentages of immunosuppressive Treg and type-1 regulatory (Tr1) cells were also determined. In both BM and PB samples, IL-10 mRNA expression was higher in metastatic NB patients than in controls. IL-10 plasma concentration was higher in patients with NB regardless of stage. Neither IL-10 expression nor IL-10 plasma concentration significantly associated with patient survival. In PB samples from metastatic NB patients, ARG-1 and CD163 expression was higher than in controls but their expression did not associate with survival. Moreover, ARG-1 plasma concentration was lower than in controls, and no association with patient outcome was found. Finally, in metastatic NB patients, the percentage of circulating Treg was higher than in controls, whereas that of Tr1 cells was lower. In conclusion, although IL-10 concentration and Treg percentage were increased, their contribution to the natural history of metastatic NB appears uncertain.