Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity

Figure 2

Experimental design of immunization with purified antigens and different adjuvants. IgGs, from naturally surviving ICR mice to PyL infection, were purified and used to isolate blood-stage parasite antigens. BALB/c mice divided into 6 groups were then inoculated with 2 μg of purified antigens using CpG ODN₁₈₂₆ and/or Freund’s adjuvant systems or with proper controls on days 1 and 27 and infected with 2 × 10⁷ PyL-RBCs on day 55.