Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity

Figure 3

Course of infection after vaccination with different adjuvants in BALB/c mice. Mice were divided into 6 groups ( each) to be immunized on days 1 and 27. Group (G) 1 with 2 μg of purified antigens and CpG ODN₁₈₂₆ plus IFA, G2 with 2 μg antigens plus CFA, G3 with 2 μg of antigens, G4 with PBS and CpG ODN₁₈₂₆ plus IFA, G5 with PBS and CFA, and G6 with PBS. On day 27 CFA was replaced with IFA. At day 55, all mice were challenged with 2 × 10⁷ PyL-RBCs. Percentages of (a) survival and (b) parasitemia are shown as mean (± SEM). comparing to controls with antigens or same adjuvant alone or PBS (G2 versus G3, G5, and G6; G1 versus G3, G4, and G6).

(a)

(b)