Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity

Figure 4

Experimental design of immunization with purified antigens and Freund’s adjuvant. Blood-stage parasite antigens were isolated by using IgGs from surviving ICR mice to PyL infection and were inoculated together with Freund’s adjuvant in BALB/c mice on days 1, 25, 50, and 85. Two weeks later mice were infected with 2 × 10⁷ PyL-RBCs. Mouse sera obtained on days 40, 70, and 100 were subjected to immunoblot analysis.