FoxP3 Tregs gating strategy. Example result obtained for a single patient before ASIT. PBMC was stained with combination of surface antigens (anti-CD4, anti-CD25, and anti-CD127) and intracellularly with anti-FoxP3. For analysis, CD4 positive cells from PBMC were gated ((a) dot plot SS versus CD4, gate B) and analyzed for CD25 and FoxP3 expression ((b) dot plot CD25 versus FoxP3 for B gate); cells in H gate were considered Tregs. The expression of CD127 in Tregs population was analyzed ((c) dot plot CD4 versus CD127 for H gate).