Respiratory Syncytial Virus Nonstructural Proteins Upregulate SOCS1 and SOCS3 in the Different Manner from Endogenous IFN Signaling

Figure 1

Identification and expression of the reconstituted plasmids pNS1, pNS2, and pNS1/2. (a) DNA gel electrophoresis analysis of the expression plasmids. pcDNA3.1(+) plasmids inserted with NS1, NS2, or NS1/NS2 artificial ORFs were digested with enzymes for 30 min, and the products were separated in a 1% agarose gel. (b) Total cellular extracts were collected from A549 cells at 2 h after plasmid transfection with final concentrations of 0, 1, 5, 10, or 20 μg/mL, and Western blot was performed to measure protein levels. The data are shown as fold increases in protein density compared with the concentration of 20 μg/mL, assumed to be 1.0.

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