Respiratory Syncytial Virus Nonstructural Proteins Upregulate SOCS1 and SOCS3 in the Different Manner from Endogenous IFN Signaling

Figure 4

RSV NS proteins decreased the induction of IFN-α-induced antiviral genes through SOCS1 expression. A549 cells were treated with 5000 U/mL IFN-α for 30 min and were then transfected with pNS1 or pNS2 for the indicated times. Cellular total protein lysates were subjected to Western blot analysis of SOCS1 (a). The data were normalized to the level of β-actin and shown as the relative density from three independent experiments. versus IFN-α. Total RNA was subjected to qPCR. The cDNA levels were analyzed using qPCR to assess the mRNA amounts of MxA or 2,5-OAS1 (b, c). Equivalent mRNA amounts were normalized to GAPDH mRNA levels and calculated as -fold changes compared with the levels of untreated cells at the respective time points. The results shown are representative of three independent experiments.

(a)

(b)

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