Expansion of Circulating T Follicular Helper Cells in Children with Acute Henoch-Schönlein Purpura

Figure 2

Increased percentages of circulating CXCR⁺CD4⁺TFH cells in the peripheral blood of HSP patients. Peripheral blood mononuclear cells (PBMC) from 22 patients with ITP and 12 healthy controls (HC) were isolated and stained with labeled antibodies and analyzed by flow cytometry as described in Section 2. The cells were gated initially on lymphocytes (a) and then on CD4⁺T cells (b). (c) ICOS⁺CXCR5⁺TFH cells in the total CD4⁺T cells of HC and HSP patients. (d) PD-1⁺CXCR5⁺TFH cells in the total CD4⁺T cells of HC and HSP patients. (e), (f), and (g) The analysis of percentages of CXCR5⁺CD4⁺T cells, ICOS⁺CXCR5⁺CD4⁺T cells, and PD-1⁺CXCR5⁺TFH cells from HCs and HSP patients. Each data pointer presents an individual subject. The horizontal lines show means.