Heparin Interaction with the Primed Polymorphonuclear Leukocyte CD11b Induces Apoptosis and Prevents Cell Activation

Figure 3

((a), (b)) Early and total apoptosis (resp.) in PMNLs separated from NC (▲) and HD (△) after incubation with 100 U/mL of heparin for 300 min. The results of NC (●) and HD (○) represent the apoptotic levels throughout 300 min without heparin. Apoptosis was determined by flow cytometry using specific FITC-labeled Annexin-V, as described in Section 2. Data are expressed as percentage of apoptotic PMNLs; . (c) PMNL count using trypan blue exclusion assay: NC and HD PMNLs were incubated with 0 and 100 U/mL of heparin for 0, 30, and 90 minutes. Data is expressed as cells counts; .

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