Automatic Generation of Validated Specific Epitope Sets

Figure 3

Predicted epitope pools induce a detectable ex vivo cytokine response. PBMC from donors with LTBI (MTB donor) or previously exposed to DENV were stimulated with their respective epitope pools and an EBV epitope pool. After ex vivo stimulation, the IFNγ response was measured by ICS using flow cytometry. Gating strategy (a) and representative plots of the responses for both CD4⁺ and CD8⁺ T cells are shown for a MTB donor (b) and DENV donor (c). (d) The ICS IFNγ responses induced by each epitope pool for all donors tested are summarized (). Statistical difference was determined using a one-tailed Mann-Whitney test. (; ).

(a)

(b)

(c)

(d)