The Peptide Vaccine Combined with Prior Immunization of a Conventional Diphtheria-Tetanus Toxoid Vaccine Induced Amyloid β Binding Antibodies on Cynomolgus Monkeys and Guinea Pigs
Induction of the anti-Aβ antibodies and Aβ peptides in the peripheral blood by immunization of the peptide vaccine to cynomolgus monkeys. The serum anti-Aβ antibody levels of the cynomolgus monkeys treated with vehicle or RGD-DiTox20-KK-Aβ1–13 (a). The plasma Aβ40 and Aβ42 peptide (b) level (nM) of cynomolgus monkeys. Diphtheria and tetanus toxoids (DT, 0.5 mL/head, s.c.) were administered to cynomolgus monkeys. Three weeks after the DT treatment, vehicle or the peptide (0.5 or 2.5 mg/head, s.c.) was administered at intervals of 2 weeks (arrows; total 9 times). Blood sampling was performed every week after the initial treatment. Results are represented as mean ± SE (). and as compared with the vehicle control group (the Dunnett test). Epitope-mapping of plasma anti-Aβ antibodies immunized with vehicle or RGD-DiTox382–401-KK-Aβ1–13 peptide (c). Epitope-mapping of antibodies was performed using each peptide-precoated ELISA with plasma collected at two weeks after the final treatment. Results are represented as mean ± SE (). KK-Aβ1–13: RGD-DiTox20-KK-Aβ1–13 peptide.
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