The Peptide Vaccine Combined with Prior Immunization of a Conventional Diphtheria-Tetanus Toxoid Vaccine Induced Amyloid β Binding Antibodies on Cynomolgus Monkeys and Guinea Pigs
Induction of the anti-Aβ antibodies in the plasma and Aβ peptides in the brain by immunization of the peptide vaccine to guinea pigs. Epitope-mapping of plasma anti-Aβ antibodies immunized with RGD-DiTox382–401-KK-Aβ1–13 peptide (a). Epitope-mapping of antibodies was performed using each peptide-precoated ELISA with plasma collected at two weeks after the final treatment. Results are represented as mean ± SE. . The plasma anti-Aβ antibody levels of the guinea pigs treated with RGD-DiTox20-KK-Aβ1–13 peptides and the correlation of anti-Aβ antibody concentrations to Aβ40 and Aβ42 peptide doses in the plasma (b). The Aβ peptide levels in the brain of guinea pigs (c and d). Diphtheria and tetanus toxoids (DT, 200 μL/head, s.c.) were administered to guinea pigs. Three weeks after the DT treatment, vehicle or the peptide (200 μg/head) was administered at intervals of 3 weeks (arrows; total 6 times). Blood sampling was performed every week after the initial treatment. Results are represented as mean ± SE (). and as compared to the vehicle control group (the Dunnett test).
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