The Peptide Vaccine Combined with Prior Immunization of a Conventional Diphtheria-Tetanus Toxoid Vaccine Induced Amyloid β Binding Antibodies on Cynomolgus Monkeys and Guinea Pigs

Figure 2

Induction of the anti-Aβ antibodies in the plasma and Aβ peptides in the brain by immunization of the peptide vaccine to guinea pigs. Epitope-mapping of plasma anti-Aβ antibodies immunized with RGD-DiTox_382–401-KK-Aβ_1–13 peptide (a). Epitope-mapping of antibodies was performed using each peptide-precoated ELISA with plasma collected at two weeks after the final treatment. Results are represented as mean ± SE. . The plasma anti-Aβ antibody levels of the guinea pigs treated with RGD-DiTox₂₀-KK-Aβ_1–13 peptides and the correlation of anti-Aβ antibody concentrations to Aβ₄₀ and Aβ₄₂ peptide doses in the plasma (b). The Aβ peptide levels in the brain of guinea pigs (c and d). Diphtheria and tetanus toxoids (DT, 200 μL/head, s.c.) were administered to guinea pigs. Three weeks after the DT treatment, vehicle or the peptide (200 μg/head) was administered at intervals of 3 weeks (arrows; total 6 times). Blood sampling was performed every week after the initial treatment. Results are represented as mean ± SE (). and as compared to the vehicle control group (the Dunnett test).

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