Gene Expression Profile of Dendritic Cell-Tumor Cell Hybrids Determined by Microarrays and Its Implications for Cancer Immunotherapy

Figure 1

cDNAs generated from DC-DC hybrid cells (DC-DC), D5lacZ tumor cell hybrids (T-T), and DC-tumor cell hybrids were subjected to quantitative real-time PCR analyses using primer pairs which detect the Th1 cytokines IFN-γ, TNF-α, IL-2, IL-12p40, IL-15, and IL18 and the Th2 cytokines IL-4, IL-10, IL-13, and IL-25. Samples were run in duplicate and difference in mRNA expression was calculated using the ΔΔCt method. Error bars represent the standard error of the mean. One representative experiment out of three independent experiments is shown.