Spontaneous Intestinal Tumorigenesis in Mice Requires Altered T Cell Development with IL-17A

Figure 3

The heterozygous Apc gene mutation in Treg alone does not impair Treg-mediated tumor regression. (a–c) 4 × 10⁵ CD4+Foxp3+ cells were isolated from 6-7-week-old -FIR mice, their wild type littermate controls (FIR mice), and Foxp3-Cre; mice by cell sorting and then transferred to 3-month-old mice. Tumor numbers were measured 4 weeks after adoptive transfer. Large tumors (>2.0 mm) and small tumors (0.5–2.0 mm) were counted. (d) Tumors (>2.0 mm) from each group of mice were excised and analyzed by real time PCR. Each group had more than 5 polyps analyzed. mRNA expression was normalized against HPRT for their relative expression level (HPRT = 1). (e–g) 4 × 10⁵ Tregs from 6-7-week-old IL-17A deficient (KO)- mice were transferred to 3-month-old mice ( per group). Tumor numbers were measured 4 weeks after adoptive transfer. Data are shown as mean ± SEM. For (a–c), one-way ANOVA analysis with Bonferroni’s post hoc test was used. For other analyses, two-tailed -tests were performed for all groups. ; ; ; n.s.: not significant.

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